Composite

Part:BBa_K1893010:Design

Designed by: Akash Bhattacharjee, Gear Rotrattanadumrong   Group: iGEM16_Imperial_College   (2016-10-14)


Constitutive STAR (J23119+STAR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end.

Source

The pAD1.A5 STAR sequence (STAR-antisense)

References

Meyer, S., Chappell, J., Sankar, S., Chew, R. and Lucks, J. (2015). Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies. Biotechnol. Bioeng., 113(1), pp.216-225.